منابع مشابه
Rapid, simplified method for production and purification of tetanus toxin.
A rapid, simplified method for production and purification of tetanus toxin from bacterial extracts was described. The extracts were prepared by stirring young cells (ca. 45-h culture) of Clostridium tetani in 1 M NaCl-0.1 M sodium citrate, pH 7.5, overnight at 0 to 4 degrees C. The toxin was purified by a combination of (i) ammonium sulfate fractionation (0 to 40% saturation), (ii) ultracentri...
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High-titered yields of human cytomegalovirus (CMV), strain AD 169, were produced in WI-38 cells in large roller bottles. Maximum plaque titers were observed by the 4th day after infection at which time infectivity in the medium was 200 times greater than that associated with the cells. Virus released into the medium was recovered by sedimentation in a sucrose gradient in a continuous-flow centr...
متن کاملHigh-level expression of tetanus toxin fragment C in Escherichia coli
Fragment C is the C-terminal domain of the heavy chain of tetanus toxin that can promote the immune response against the lethal dose of this toxin. Therefore, this portion can be considered as a candidate vaccine against tetanus infection, which occurs by Clostridium tetani. The present study aimed to compare the expression of tetanus toxin fragment C in Escherichia coli BL21 (DE3) pLysS cells...
متن کاملHigh affinity binding of tetanus toxin to mammalian brain membranes.
A sensitive binding assay was developed to study tetanus toxin binding to brain membranes in vitro. Binding of '2"I-tetanus toxin was linear with tissue content only at very dilute tissue concentrations, 0-20 ng membrane protein/200 pl for bovine cortex membranes. At these low tissue concentrations '261-tetanus toxin displayed high affinity, single phase binding with a dissociation constant (KD...
متن کاملStructure of Tetanus Toxin DEMONSTRATION AND SEPARATION OF A SPECIFIC ENZYME CONVERTING INTRACELLULAR TETANUS TOXIN
Protease activity has been demonstrated in culture supernatants of Clostridium tetani at various stages of fermentation. Gel chromatography of the concentrated filtrates revealed the presence of three enzymatically active fractions eluting at separate positions off the column. The smallest protease was found to “nick” the single chain intracellular tetanus toxin, producing the extracellular, tw...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1945
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.49.5.515-516.1945